Scientific Approach

1. Most cytosolic protein lack disulphide, whereas extracellular protein usually contain them. Why?
2. Calculate the pH of a mixture of 0.25 M acetic acid and 0.1 M, sodium acetate and PKa of acetic acid is 4.76.,
3. A peptide was broken into two smaller peptide by CNBr and two different peptide by Trypsin. There sequence were as follows:--

a. CNBr 1 – Gly – Thr – Lys – Glu
b. CNBr 2 – Ser – Met
c. Trp 1 – Ser – Met – Gly – Thr – Lys
d. Trp 2 – Ala – Glu
4. A sample of purified DNA obtained from tobacco leaf contain 20 mole percent of cytosine. Assuming that only the four principal bases are present. Calculate the approximate mole percent of Purine residue in this DNA.
5. Lysine is an amino acid with ionizable group. These are the a-COOH, a-amine group and e-amino group with pKa value of 2.2, 9.2 and 10.8 respectively. What is the isoelectric point for lysine?

Section-B
6. Describe one method which you consider best for determining the C-terminal end of proteins. Give reason for your choice.
7. What are the biological role of folic acid? What are its deficiency symptoms?
8. Describe physiological properties of H2O.
9. Describe general structure and function of lipids.
10. Describe in detail the structure of Monosaccharide.
11. Write structure of Vitamin E. Describe its function.
12. Draw the outline of t-RNA. Briefly describe function in protein synthesis.
13. Describe reverse turn and Rama Chandran plot.
14. Describe the difference between DNA and RNA.
15. What do you understand by globular protein? What is their significance? 16. Write in detailed about m-RNA.
17. Describe the function of two glycolipid.
18. Describe the structure and deficiency symptom of Riboflavin.
19. Name different endocrine gland. Write in detail about insulin.
20. If galactose were present in urine to be tested, how might an anylyst erroneously report the presence of glucose instead?

Section-C
21. Give in detail one method which you consider best for the chemical synthesis of polynucleotides. Give reason for your choice.
22. Discuss the classification of vitamins. Describe the transamination, deamination, discarboxylation and recemisation reaction catalysed by Vitamin B-6.
23. Describe chemical synthesis of peptide and protein sequencing.

MOLECULAR CELL BIOLOGY

1.Select and write the correct answer from the options given for each statement.
a). The Na+/ K+ ATPase functions to:

I. move 3K + ions out of the cell and 2Na+ ions into the cell.
II. Move 2K+ ions out of he cell and 3Na+ ions into the cell
III. Move 2K+ ions out of the cell and 3K+ ions into the cell.
IV. Move 3Na+ ions out of the cell and 2K+ ions into the cell

b). which of the following amino acids would most likely to be found in a membrane spanning a helix? I. Lysine II. Glutamine III. Aspartic acid IV. Alanine V. Arginine c). The gene that acts as a negative regulator of apoptosis in c. elegans is: i. Ced-3 ii. Ced-4 iii. Ced-9 iv. Reaper d). Polo kinase phosphorylates and activates: I. Cdc6 II. Cdc20 III. Cdc25 IV. M-Cdk e). The single most abundant protein in animal tissues is: I. Fibronectin II. Collagen III. Talin IV. Laminin f). which of the following statements is not true for the intermediate filaments? I. They are made up of very different types of subunits. II. They have a very high tensile strength III. Motor proteins use filaments for movement of cargo IV. They do have intrinsic polarity g). Selectins recognize cell surface:b). which of the following amino acids would most likely to be found in a membrane spanning a helix? I. Lysine II. Glutamine III. Aspartic acid

IV. Alanine
V. Arginine
c). The gene that acts as a negative regulator of apoptosis in c. elegans is:

i. Ced-3
ii. Ced-4
iii.Ced-9
iv. Reaper
d). Polo kinase phosphorylates and activates:

I. Cdc6
II. Cdc20
III.Cdc25
IV. M-Cdk
e). The single most abundant protein in animal tissues is:

I. Fibronectin
II. Collagen
III.Talin
IV. Laminin
f). which of the following statements is not true for the intermediate filaments?

I. They are made up of very different types of subunits.
II. They have a very high tensile strength
III.Motor proteins use filaments for movement of cargo
IV. They do have intrinsic polarity
g). Selectins recognize cell surface: